Electrophoresis System with Blue Light

Product Introduction

The uniquely designed Servicebio Horizontal Electrophoresis System features a built-in blue light at the bottom of the tank, allowing clear visualization of sample bands during electrophoresis. When used with the Servicebio SPW-6S Electrophoresis Power Supply (Programmable), which includes a dedicated 12V output port, the blue light is perfectly powered—safe and convenient. With this Integrated Horizontal Electrophoresis System, you can observe sample bands in real time throughout the run, ensuring better experimental results.

Product Features

1. Integrated design combining a horizontal electrophoresis tank and a blue light transilluminator—bands can be viewed directly at any time during electrophoresis.

2. Perforated lid for heat dissipation and easy observation.

3. Removable electrode assembly and electrode tips for convenient cleaning and maintenance.

Technical Specifications

1. External dimensions (L×W×H): 310 × 150 × 120 mm

2. Gel sizes (L×W): Large gel 120 × 120 mm; Wide gel 60 × 120 mm; Long gel 120 × 60 mm; Small gel 60 × 60 mm

3. Net weight: 0.8 kg

4. Buffer capacity: 650 mL

Product Overview

SVL-2 Horizontal Electrophoresis System is mainly used for rapid agarose electrophoresis separation experiments of small amounts of DNA and RNA samples.

Product Structure

Product  Specifications

Operation Steps

Please ensure that the horizontal electrophoresis tank, gel tray, sample comb, gel maker and other components are clean and dry before gel preparation.

1. Preparation

Weigh an appropriate amount of agarose into an Erlenmeyer flask, add an appropriate amount of buffer, place it in a water bath, a magnetic heating stirrer or a microwave oven and heat until it is completely melted, shake well and make an agarose gel solution.

2.Gel Making

Place the gel maker horizontally on the experimental bench, select a suitable gel tray (different sizes of gel trays are selected according to different experimental needs, the specifications of the gel tray are 120mmX120mm , 120mmX60mm , 60mmX120mm , 60mmX60mm), put the gel tray in the gel maker (two pieces of  60mmX60mm gel can be made at the same time, and only one piece can be made for other specifications) and put the sample comb in a fixed position. Add 5ul SerRed (or other nucleic acid dyes) solution to the agarose gel cooled to about 55°C . After mixing, carefully pour it into the gel tray, and slowly spread the gel until the entire surface of the gel tray is uniform. For the gel layer, let stand at room temperature until the gel is completely solidified, and then gently pull out the sample comb vertically to complete the preparation of the gel plate.

3.Sample Loading

Take the prepared gel and the gel tray out of the gel maker together and place them in the electrophoresis box with the sample addition hole close to the negative electrode (black is the negative electrode). Add running buffer until the gel plate is covered.

Use a 10ul micropipette to add the samples to the sample grooves of the rubber plate respectively. After each sample is added, a sample addition head should be replaced to prevent contamination. When adding samples, do not damage the gel surface around the sample wells (pay attention to the order of adding samples) .

4.  Electrophoresis

Cover the top cover according to the correct position of the positive and negative poles (red is positive, black is negative), insert the electrophoresis wire into the electrophoresis power supply according to the correct color, and select the appropriate voltage to start electrophoresis (the specific electrophoresis parameters are adjusted according to the actual experimental parameters) .

The sample moves from the negative (black) to the positive (red) direction. As the voltage increases, the effective separation range of the agarose gel decreases. The electrophoresis was stopped when the bromophenol blue moved to about 1 cm from the lower edge of the gel plate.

5.  Observation

This Integrated Horizontal Electrophoresis System is equipped with a blue light light transilluminator, which can observe the electrophoresis situation in real time with SPW-M500 Electrophoresis Power Supply, SPW-6S Electrophoresis Power Supply (Programmable).

Product Configuration List

Precautions

1. Do not operate this product with electricity (the top cover of the horizontal electrophoresis apparatus must be covered before the electrophoresis power is turned on after the sample is added, and do not touch the horizontal electrophoresis apparatus during the electrophoresis process to avoid the danger of electric shock).

2. All parts of this product can be washed with water, just dry naturally or dry with absorbent paper. Do not bake at high temperature to avoid damage to the product.