CravitySci MOIS HT Small Animal In Vivo Imaging System

CravitySci MOIS HT Small Animal In Vivo Imaging System

Product Description

The CravitySci MOIS HT Small Animal In Vivo Imaging System is a highly sensitive, multimodal optical imaging platform designed for non-invasive visualization, monitoring, and quantitative analysis of biological processes in preclinical research. Integrating bioluminescence, fluorescence, and spectral high-resolution imaging technologies, the system enables researchers to investigate molecular and cellular activity in living small animals with minimal experimental disruption.

Designed for demanding low-light applications, the MOIS HT combines deep-cooled CCD detection, high-efficiency optics, advanced spectral separation, and comprehensive image optimization. Its flexible imaging capabilities support research ranging from localized signal detection to whole-body monitoring, including tumor development, immune and stem cell tracking, gene expression, drug distribution, and therapeutic response evaluation.

The integrated imaging chamber, environmental control system, and data acquisition software provide a versatile platform for multi-animal studies and repeated observations across different experimental conditions and time points.

Key Features

• Exceptional Low-Light Sensitivity: Deep-cooled CCD detection reduces dark current and thermal noise, improving the visibility of weak biological signals during sensitive optical imaging experiments.
• Multimodal Optical Imaging: Combines multiple imaging techniques to enable complementary investigation of biological activities, molecular distributions, and experimental responses.
• High-Efficiency Optical Design: A large-aperture imaging lens enhances photon collection efficiency and supports improved image detail under low-signal conditions.
• Advanced Spectral Unmixing: Separates overlapping fluorescent signals, reduces interference from tissue and animal fur, and facilitates multi-probe imaging and analysis.
• Quantitative Imaging Reliability: Calibrated light measurements and illumination uniformity correction support consistent analysis across imaging conditions and experimental sessions.
• High-Throughput Animal Monitoring: A multi-animal imaging platform allows researchers to monitor multiple experimental subjects during a single imaging session.
• Comprehensive Noise Suppression: Integrated image correction and background reduction functions improve signal discrimination, image uniformity, and experimental reproducibility.
• Longitudinal Imaging Capability: Time-series acquisition and non-invasive observation support repeated monitoring of biological processes throughout an experimental study.

Advanced Imaging Technologies

Bioluminescence Imaging

Bioluminescence imaging enables non-invasive detection of light emitted from biological reporters in living subjects. The highly sensitive optical detection system supports monitoring of tumor growth, gene expression, cellular activity, and treatment responses, including experiments requiring detection of low-intensity signals.

Fluorescence Imaging

Fluorescence imaging enables visualization of labeled cells, tissues, and molecular targets using compatible fluorescent probes. The optical system accommodates commonly used fluorescence wavelengths, supporting applications such as cell migration, molecular targeting, biodistribution, and disease-associated biomarker studies.

Spectral Unmixing and Multi-Probe Imaging

Advanced spectral unmixing technology helps resolve signal overlap between different fluorescent probes while suppressing interfering background fluorescence. This capability enables more accurate identification and localization of individual fluorescence signals within complex biological environments, supporting multi-color imaging and molecular co-localization studies.

Cerenkov Luminescence Imaging

The MOIS imaging platform is described in the product literature as supporting Cerenkov Luminescence Imaging (CLI) for radionuclide-based optical studies. CLI uses light generated through interactions associated with suitable radionuclide tracers, enabling investigation of radiotracer distribution and selected drug evaluation applications. The exact CLI module and software configuration should be confirmed for the supplied system.

Quantitative Imaging and Image Optimization

The MOIS HT incorporates optical calibration and image-processing technologies designed to improve measurement consistency and reduce unwanted background interference. Its specialized light-tight imaging chamber helps minimize external light contamination during sensitive signal acquisition.

• Absolute Bioluminescence Calibration: Uses a calibrated light reference to support quantitative comparison of bioluminescent signals under different imaging conditions.
• Excitation Uniformity Correction: Compensates for variations in illumination intensity across the imaging field to improve fluorescence measurement consistency.
• Bias and Flat-Field Correction: Reduces detector-related offsets and spatial variations to enhance image uniformity.
• Cosmic Ray Removal: Suppresses unwanted high-intensity artifacts caused by cosmic ray events during image acquisition.
• Background Suppression: Supports correction of unwanted luminescence and fluorescence backgrounds to improve the visibility of meaningful biological signals.

Imaging Workflow and Software Capabilities

The integrated acquisition software supports real-time image capture, time-series experiments, communication connectivity, and convenient switching between optical imaging modes. Synchronous acquisition functionality is also supported, enabling flexible experimental data collection.

Image analysis and time-spectral analysis tools assist researchers in interpreting experimental signals and comparing imaging results. Additional molecular quantitative analysis functions may require separate software authorization.

Multi-Animal Imaging and Experimental Support

Designed for efficient preclinical workflows, the system accommodates simultaneous imaging of multiple small animals. An integrated temperature-control environment supports controlled imaging conditions, while the multi-channel anesthesia arrangement allows inhalation anesthesia during imaging procedures.

Active anesthetic gas-scavenging masks help limit waste anesthetic gas leakage, and crosshair positioning lasers assist with accurate placement of experimental subjects. These features support consistent positioning, efficient handling, and repeated observation in longitudinal studies.

Research Examples

The manufacturer provides examples of imaging experiments and data analysis applications, including:

• Multispectral separation of FITC, Cy3, Cy5, Cy7, and ICG fluorescent probes for multi-probe imaging.
• Radionuclide distribution monitoring using Cerenkov luminescence imaging, including I-131 detection.
• Nanoparticle biodistribution analysis in harvested organs and tissues at multiple time points.
• Optical imaging of orthotopic brain metastasis mouse models.
• Biological signal monitoring in abdominal xenograft tumor models.
• Near-infrared imaging using Alexa Fluor 680-conjugated probes.
• In vitro cellular imaging and in vivo tracking with Cy5 fluorescent labels.

Applications

• Oncology Research: Tumorigenesis, tumor progression, metastasis, and treatment response monitoring.
• Immunology: Immune cell distribution, migration, and disease-associated immune response studies.
• Stem Cell Research: Cell localization, tracking, migration, and distribution analysis.
• Neuroscience: Investigation of neurological disease models, molecular activity, and brain-associated biological processes.
• Cardiovascular Research: Optical imaging applications in cardiovascular disease studies.
• Infectious Disease Research: Pathogen-associated signal monitoring and investigation of biological distribution patterns.
• Drug Development: Drug screening, biodistribution, pharmacokinetics, and therapeutic efficacy evaluation.
• Genetics and Molecular Biology: Gene expression, molecular interactions, and signaling pathway studies.
• Longitudinal Preclinical Studies: Repeated non-invasive monitoring of biological changes across multiple experimental time points.

Configuration and Ordering Notes

The MOIS HT configuration supports bioluminescence, fluorescence, and spectral high-resolution imaging. Upconversion fluorescence imaging is identified as an expandable or upgradeable capability rather than a confirmed standard module. Certain advanced analysis functions require software authorization.

X-Ray imaging is not included in the MOIS HT configuration. Availability of specialized imaging modules, associated software, additional accessories, and the final supplied configuration should be confirmed before ordering.