AAV9 Capsid Libraries

AAV9 Capsid Libraries are designed for the evolution and development of novel AAV capsids with specific characteristics based on AAV9 capsid. AAV9 Capsid Libraries have 7 to 12 amino acid random peptides insertion on the surface of viral capsid at VR-IV or VR-VIII region. AAV9 CAP gene with random peptide are driven by a CAG/P40 hybrid promoter. P40 promoter was used to drive CAP expression during AAV production. CAG promoter is used to express CAP in targeting cells or tissues.

• SKU: DB005-AAV9
• AAV Capsid Library: ATHENA-II random peptide insertion
• Library Titer: 1e13 VG/ml
• Promoter:  CAG/P40
• AAV serotype: AAV9
• Insert Region(site): VR-IV(S454_G455) or VR-VIII(Q588_A589)
• Insert Random Peptide Length: 7 amino acids
• Complexity: >1e9
• Production Condition: 200 copies/cell
• Production Cell Line: HEK 293T
• Purification Method: Two rounds of CsCl ultracentrifuge
• Storage Buffer: 0.001% F-68/DPBS with additional 150mM NaCl.
• Storage:  -80°C for long term (> 1 year);  -20°C for short term(1-2 months);  4 °C for 1-2 weeks.
• Shipping: Dry ice.

• Insert Region Option:

• VR-IV
• VR-VIII

• Volume OPTIONS:

• 1000 uL
• 200 uL
• 500ul
• 100 uL